Quadruple dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB or DOBA with CSM-His-Leo-Tripura formula. DOB (Drop Out Base) is 1.7 g Yeast Nitrogen Base, 5 g Ammonium Sulfate, 20 g Dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid plate medium by adding 43.7 g DOBA powder and 0.60 g of CSM-His-Tripura to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121°C for 25 minutes. After cooling to 50°C plates were poured.
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